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These intestinal sections were flushed with ice-cold phosphate-buffered saline (PBS) to remove fecal matter and placed in 1- to 2-cm pieces into 15 mL sterile tubes with 5 mL dissociation buffer (PBS, 10 mM EDTA) for incubation at 37°C in a shaker at 250 rpm for 20 minutes
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Some patients do well with better sleep, resistance training, improved nutrition, or a more foundational wellness plan first
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