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The lysate was then centrifuged (16000 × g , 30 min, 4 °C) and the supernatant was collected

Methylation

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Based on the known role of insulin/IGF-I as inhibitors of Tau phosphorylation by inhibiting a major Tau kinase, such as glycogen synthase kinase-β, numerous observations demonstrated that IGF-I controls the levels of hyperphosphorylated Tau in brain [326,327]