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Some common causes of developing under-eye hollows include: Aging - Loss of fat pads and thin skin contribute to hollows as you grow older

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Sections were then incubated in biotinylated goat anti-rabbit antibody (Abcam #ab6720, RRID: AB_954902, 1:500) for 2 h, washed three times, and incubated with horseradish peroxidase (HRP)-conjugated Streptavidin (Vector Laboratories #SA-5004-1, 1:500 in PBS) for 2 h