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Standard 1X PBS is a tightly defined formulation of 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, and 1.8 mM KH₂PO₄ at pH 7.4 , designed to create an isotonic environment of about 280 to 300 mOsm/L that matches physiological conditions and supports cell viability during washing, dilution, and transport, as outlined in this detailed guide to PBS solution
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