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For relative expression analysis, the comparative 2 −(ΔΔCt) method was applied to normalise IFNG expression to the endogenous EF1A reference and to express modulation as the relative FC to PBS controls

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Fatty acid metabolism affects neuroimmune microenvironment by regulating signaling pathways like PPARs (Peroxisome Proliferator-Activated Receptors), NF-κB (Nuclear factor kappa-light-chain-enhancer of activated B cells), and Nrf2 (Nuclear factor E2-related factor 2), influencing the functions of neural and immune cells (16–18)