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To cope with the elevated oxidative stress they generate, cancer cells enhance their antioxidant capacity, largely through sustained activation of the transcription factor NRF2 often driven by mutations in its negative regulator KEAP1
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SYBR Premix Ex Taq (Takara Bio, Shiga, Japan) was used for qRT-PCR and the condition of the 40 reaction cycles was controlled by a Corbett Research Rotor-Gene 3000 (QIAGEN Korea Ltd., Heiden, Germany) as follows: 95°C for 10 seconds, 59°C for 15 seconds, and 72°C for 20 seconds