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MC4R variants, FTO polymorphisms, and ASIP genes influence baseline melanin production and response to depigmenting agents

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Proteins in total homogenates (20–40 μg) or nuclear extracts (40 μg) were resolved by 10% SDS-PAGE and immunoblotted using following primary antibodies: chicken polyclonal antibodies against mouse GCLM 7 and mouse ALDH1A1 60