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We washed the membrane 3 times in TBS containing 0.1% Tween 20 and incubated with secondary antibodies HRP-conjugated Goat anti-Mouse IgG (1:10,000, Santa Cruz Biotechnology, sc-2005) and HRP-conjugated Goat anti-Rabbit IgG secondary antibody (1:10,000, Santa Cruz Biotechnology, sc-2004) for 1 hour at room temperature
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However, studies have reported mixed results, and current evidence is insufficient to conclude that glutathione supplementation consistently improves recovery, reduces muscle soreness, or enhances athletic performance