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Cells were then washed three times in HBSS, at room temperature, with centrifugation at 250 g for 5 min, once the washes were completed cells were resuspended in HBSS and incubated at room temperature for 10 min to allow de-esterification of AM ester by intracellular esterase

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In the present review, we firstly summarized the studies on GH-IGF1 signaling in the development of cancers