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Cell Culture Immortalized mouse hippocampal cell line HT22 was cultivated in high-glucose DMEM (C11995500BT, Gibco, United States) containing 10% FBS (10270-106, Gibco, United States), 100 U/ml penicillin and 100 μg/ml streptomycin that maintained in a 5% CO 2 incubator at 37°C