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We propose an in vitro study to measure cell proliferation as well as EGF signaling activity in the presence of ATZ alone, EGFR antagonist alone, curcumin alone, ATZ and EGFR antagonist co-treatment, and ATZ and curcumin co-treatment

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GPCRs couple to intracellular, GTP-binding, heterotrimeric G proteins that consist of Gα, Gβ, and Gγ subunits (Figure 1)