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Waltham, MA, USA) staining as adapted and modified from published protocols.[12] Briefly, cells were washed twice with PBS, fixed with 10% formalin for 30 min at room temperature, and stained with oil red O working solution (~0.2% oil red O in 99% isopropanol) for 30 min
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In a 12-week trial, adults on AOD-9604 lost about 2.6 kg versus 0.8 kg on placebo, and the larger, adequately powered trial did not beat placebo
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