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The samples were heated to 90 °C for 10 min in boiling water and the protein precipitate formed were pelleted by centrifugation
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Importantly, numbers of Olig2+ (Fig 7D) and GST3+ (Fig 7E) cells were significantly higher in the combined 78c group (which includes LL-RD-78c and LL-HFD-78c mice) versus the combined group without 78c treatment (which includes LL-RD and LL-HFD)
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