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Immediate effect on the blood vessels presentation upon fistula creation was assessed considering the point immediately before therapy (point ‘0’ as 100%) and scoring the vessel presentation (recorded filled/appearance or emptied/ disappearance (camera attached to a USB microscope (Veho discovery VMS-004 deluxe, Veho, Southampton, UK))) at the 30 mm duodenum segment aboral and oral to the fistula, arcade vessels arising from the superior anterior pancreaticoduodenal vein and artery on the ventral duodenum side as described before (34-36), and a corresponding colon segment, after 3, 6, 9, 12, and 15 min

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Subsequently, the tissues were cut open along one side and laid flat on glass slides