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(18)纯化 (18) Purification 将步骤(17)的BPC157肽粗品1.23g溶于13ml水中，过滤，滤液上样于C 18 色谱柱(Kromasil-C 18 ，20mm*250mm，汉邦科技色谱公司)，流动相为如下A、B液体的混合物：A：0.1％三氟乙酸加99.9％水；B：0.1％三氟乙酸加99.9％乙腈。从A液体占混合物比例100％开始梯度洗脱，期间B液体占混合物比例逐渐升高，直至占100％，流速为10ml/min，检测波长为：215nm。收集目标峰(用相同条件下以实验室级别生产的少量纯品做对照指示目标峰)的流出液，浓缩冻干，获得成品，共获得成品0.69g(MW：1419.6)，经HPLC测定纯度大于98％。 Dissolve 1.23 g of the crude BPC157 peptide in step (17) in 13 ml of water, filter, and load the filtrate on a C 18 chromatographic column (Kromasil-C 18 , 20mm*250mm, Hanbang Technology Chromatography Company), the mobile phase is as follows A, B Mixture of liquids: A: 0.1% trifluoroacetic acid plus 99.9% water
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