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The 66 remaining children who completed the study consisted of 37 boys (77%) and seven girls (23%) (mean age: 57.23 ± 15.06 months) in the intervention group and 17 boys (77%) and five girls (23%) (mean age: 51.75 ± 12.72 months) in the control group

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High-performance LC-MS/MS was used to perform high-sensitivity, wide-coverage, and high-throughput HM700-targeted quantification of 700 metabolites in these samples ( In detail, appropriate amounts of experimental or quality control (QC) samples were resuspended in 140 μL of a 50% water/methanol solution, lysed, and centrifuged, and the supernatant was transferred to a new tube