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The supernatant was added to protein A-Sepharose beads (Life Technologies) carrying prebound anti-HA antibodies (clone 12CA5, kindly provided by Hans-Peter Hauri)
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One of the most abundantly expressed genes in ovarian tumours and tumour cell lines was glutathione S -transferase P1 ( GSTP1 , EC 2.5.1.18), a polymorphic phase II drug-metabolising enzyme, which conjugates the antioxidant tri-peptide glutathione with many toxic hydrophobic and electrophilic xenobiotics to facilitate elimination (reviewed by Hayes and Pulford, 1995)
