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These include: Large disc extrusions or sequestered fragments requiring surgical intervention Severe spinal stenosis with significant canal narrowing Bone spurs (osteophytes) compressing nerve structures Advanced degenerative disc disease with complete disc height loss Spondylolisthesis (vertebral slippage) beyond grade 1 Cauda equina syndrome (medical emergency requiring immediate surgery) Users with these conditions typically report minimal improvement from peptide therapy alone

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Aliquots A and B were incubated in 6-well plates for 24 hours at 37°C in a humidified atmosphere with 5% CO 2 ( Figure 2 )